STUDY OF PLANT ORIGIN COMPOUNDS WITH POTENTIAL ANTIFUNGAL ACTION AGAINST PATHOGENS OF CULTIVATED PLANTS
Paula Badaracco 1 *, Maximiliano Sortino 2 , Rosanna N. Pioli 1, 3, 4
Pathogenic fungi generate crop diseases, and affect yield and food production. The use of phytosanitary products to control fungal diseases can pose a risk to human health and the environment. In this context, the use of plant metabolites as potential bio-controls for pathogens turns out to be a sustainable alternative. The objective of this study was to evaluate the anti-fungal action of six compounds of plant origin: daidzein, genistein, two chalcones, vanillin and isovanillin, on the in vitro growth of pathogens. The Minimum Inhibitory Concentration (MIC) and the Minimum Fungicidal Concentration (CFM) of each chemical compound were evaluated by the broth micro-dilution method against five fungal genera: Alternaria sp., Cladosporium sp., Fusarium (F. graminearum, F. subglutinans, F. proliferatum, F. oxysporum), Phomopsis longicolla, and Rhizopus stolonifer. The results indicated that daidzein, genistein, iso-vanillin and CH1 did not show an inhibitory and / or fungicidal action at concentrations lower that 250 µg mL-1. CH2 was characterized by inhibiting four isolates belonging to three genera in different concentrations, and presenting fungicidal activity on two of them. Vanillin proved to be effective to inhibit and / or eliminate all of the isolates (nine) included in the five fungal genera evaluated at concentrations below 250 µg mL-1. It is concluded that metabolites of plant origin such as vanillin and CH2 presented antifungal capacity in different degrees and specificity on the isolates.
Key words: Plant metabolites, phytopathogenic fungi, antifungal action.
1 Botánica Criptogámica y Laboratorio Biodiversidad Vegetal y Microbiana, Facultad de Ciencias Agrarias, Universidad Nacional Rosario, Campo Experimental José Villarino, C.C. 14 (S2125ZAA), Zavalla, Argentina.
2 Farmacognosia, Facultad de Ciencias Bioquímicas y Farmacia, Universidad Nacional de Rosario, Suipacha 531 (S2002LRK), Rosario, Argentina. firstname.lastname@example.org
3 Instituto de Investigaciones en Ciencias Agrarias de Rosario (IICAR, CONICET-Universidad Nacional de Rosario), Campo Experimental José Villarino, C.C. 14 (S2125ZAA) Zavalla, Santa Fe, Argentina.
4 Consejo de Investigaciones de la Universidad Nacional de Rosario, Maipú 1065 (2000EKF) Rosario, Santa Fe, Argentina. email@example.com
*Autor para correspondencia E-mail: firstname.lastname@example.org