A REGENERATION PROTOCOL OF Calibrachoa var. PAMPA SALMÓN INTA FROM APICAL MERISTEMS

Leticia Tombion^1*, Adriana Kato¹ y María Silvina Soto¹ 

ABSTRACT

Calibrachoa, which belongs of the Solanaceae family, is a genus native to South America. Some commercial varieties of this plant have a high impact on the ornamental plant market because of the color and attractive shape of their flowers as well as their adaptability to be grown in flower beds and flower pots. The objective of this work was to develop an efficient protocol for in vitro propagation of Calibrachoa var. Pampa Salmon INTA from apical meristems. The combination of MS ½ and 0.01 mg L-1 BAP resulted in a high percentage of regeneration of plants in addition to a greater aerial (shoot) and radical (root) production that, consequently, allowed for a high rate of plant survival after transfer to the greenhouse.

Key words: Calibrachoa, ornamental plant, meristem culture, micropropagation, BAP, bencilaminopurine.

¹ Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Floricultura, 1686, Hurlingham, Buenos Aires, Argentina.

*Autor para correspondencia E-mail: tombion.leticia@inta.gob.ar